Recombinant His Tag anticorps

N° du produit ABIN5668264

Cet anticorps anti-His Tag est un anticorps Souris Monoclonal détectant His Tag dans WB, ELISA et FACS. Adapté pour .

Aperçu rapide pour Recombinant His Tag anticorps (ABIN5668264)

Antigène: His Tag

Type d'anticorp: Recombinant Antibody

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 3D5

Détail du produit anti-His Tag anticorps

Fonction: Anti-His tag (C-term) [3D5], Mouse IgG2c, kappa

Specificité: This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: Purified his tagged Fab fragment.

Isotype: IgG2c kappa

Information d'application

Indications d'application: This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997, PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015, PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019, PMID: 30885301).

Commentaires:

This is a reformatted mouse IgG2c antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag

Sujet: Polyhistidine tag